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The authors describe the unusual case of subungual onychomycosis, due to fl uconazole and itraconazole resistant Candida albicans after using the hybrid and acrylic lacquers and nail tips. The etiology of these atypical changes was supported by isolation of the fungus from the nail lesions, and its consistent identifi cation by means of morphological and molecular diagnosis. In the presented case, topical treatment with ciclopirox 8% nail lacquer allow to fi ght the pathogenic fungus but did not restore the natural appearance of the nails.
Intraspecific functional and genetic diversity of Petriella setifera (2018)
The aim of the study was an analysis of the intraspecific genetic and functional diversity of the new isolated fungal strains of P. setífera. This is the first report concerning the genetic and metabolic diversity of Petríella setífera strains isolated from industrial compost and the first description of a protocol for AFLP fingerprinting analysis optimised for these fungal species.
Metabolic and Genetic Properties of Petriella setifera Precultured on Waste (2018)
Although fungi that belong to Petriella genus are considered to be favorable agents in the process of microbial decomposition or as plant endophytes, they may simultaneously become plant pests. Hence, nutrition factors are supposed to play an important role.
Community-level physiological profiles of microorganisms inhabiting soil contaminated with heavy metals (2018)
The aim of the study was to assess the differences in the bacterial community physiological profiles in soils contaminated with heavy metals versus soils without metal contaminations. The study’s contaminated soil originated from the surrounding area of the Szopienice non-ferrous metal smelter (Silesia Region, Poland).
Występowanie drobnoustrojów rodzajów Salmonella i Listeria w mięsie ślimaków jadalnych (2018)
The objective of the research was to determine the occurrence of microorganisms from the Salmonella spp. and Listeria spp. in raw and frozen (cooked) snail meat obtained from both, free-living population and farmed edible snails. The research material comprised the meat samples (25g each) collected from three snail species, i.e. Roman snail (Helix pomatia — HP), small brown garden snail (Cornu aspersum aspersum — CAA) and large brown garden snail (Coma aspersum maxima —CAM).
Microbial biodiversity of meadows under different modes of land use: catabolic and genetic fingerprinting (2017)
The main goal of the study was to find differences in the bacterial community structure resulting from different ways of meadow management in order to get the first insight into microbial biodiversity in meadow samples. The next generation sequencing technique (454-pyrosequencing) was accompanied with the community level physiological profiling (CLPP) method in order to acquire combined knowledge of both genetic and catabolic bacterial fingerprinting of two studied meadows (hayland and pasture).
Benefits of flavonoids and straw mulch application on soil microbial activity in pea rhizosphere (2017)
The importance of flavonoids in rhizosphere–legumes symbiosis has been recognized as critical. However, the limited data are available about their impact on soil microbial communities. In rhizosphere, it remains unclear whether flavonoids, mulch or their joint effects influence on soil microbial diversity and enzymes activity.
Efficient Cellulases Production by Trichoderma atroviride G79/11 in Submerged Culture Based on Soy Flour-Cellulose-Lactose (2017)
The microbiological hydrolysis of lignocellulose waste materials, using enzymatic biopreparations and its anaerobic processing, is a promising strategy for the efficient use of renewable energy sources. This article presents the optimization of microbiological media to improve cellulase production by Trichoderma atroviride G79/11 for further application as a cellulolytic biopreparation. The characterization of T. atroviride phenotypic microarrays was performed using the Biolog® PM plates approach.
Microbial functional diversity and enzymatic activity of soil degraded by sulphur mining reclaimed with various waste (2017)
The aim of the study was to evaluate microbial functional diversity based on community level physiological profiling and b-glucosidase activity changes in soil degraded by sulphur mining and subjected to reclamation with various waste. The experiment was set up in the area of the former ‘Jeziórko’ Sulphur Mine (Poland), on a soilless substrate with a particle size distribution of slightly loamy sand.
Microbial community diversity and the interaction of soil under maize growth in different cultivation techniques (2017)
Soil microbial functional diversity under maize grown in different agricultural management practices was determined using the Biolog EcoPlates and other microbial and biochemical methods. Comparisons of substrate utilization and the diversity indices showed differences in community composition of microorganisms related to different cultivation techniques and seasons.
Comparison of Chemical Sensitivity of Fresh and Long-Stored Heat Resistant Neosartorya fischeri Environmental Isolates Using BIOLOG Phenotype MicroArray System (2016)
Spoilage of heat processed food and beverage by heat resistant fungi(HRF) is a major problem for food industry in many countries. Neosartorya fischeri is the leading source of spoilage in thermally processed products. Its resistance to heat processing and toxigenicity makes studies about Neosartorya fischeri metabolism and chemical sensitivity essential. In this study chemical sensitivity of two environmental Neosartorya fischeri isolates were compared.
Functional Diversity and Microbial Activity of Forest Soils that Are Heavily Contaminated by Lead and Zinc (2016)
The objective of this study was to assess the impact of metal contamination on microbial functional diversity and enzyme activity in forest soils. This study involved the evaluation of the influence of the texture, carbon content and distance to the source of contamina- tion on the change in soil microbial activity, which did not investigate in previous studies.
Linking Microbial Enzymatic Activities and Functional Diversity of Soil around Earthworm Burrows and Casts (2016)
The aim of this work was to evaluate the effect of earthworms (Lumbricidae) on the enzymatic activity and microbial functional diversity in the burrow system [burrow wall (BW) 0–3 mm, transitional zone (TZ) 3–7 mm, bulk soil (BS) > 20 mm from the BW] and cast aggregates of a loess soil under a pear orchard. The dehydrogenase, β-glucosidase, protease, alkaline phosphomonoesterase, and acid phosphomonoesterase enzymes were assessed using standard methods
Fast and Accurate Microplate Method (Biolog MT2) for Detection of Fusarium Fungicides Resistance/Sensitivity (2016)
The need for finding fungicides against Fusarium is a key step in the chemical plant protection and using appropriate chemical agents. Existing, conventional methods of evaluation of Fusarium isolates resistance to fungicides are costly, time-consuming and potentially environmentally harmful due to usage of high amounts of potentially toxic chemicals.
Genetic and Metabolic Intraspecific Biodiversity of Ganoderma lucidum (2015)
Fourteen Ganoderma lucidum strains from different geographic regions were identified using ITS region sequencing. Based on the sequences obtained, the genomic relationship between the analyzed strains was determined.
Soil microbial functionality in response to dairy sewage sludge and mineral fertilisers application under winter rape (2015)
Microbial activity and the diversity of their catabolic potential would be stratified according to soil profile as a result of differing content of soil organic C and they would be altered by applying dairy sewage sludge (DSS) to the surface and subsequently ploughing. We applied 26 Mg ha -1 of DSS and the same nutrient dose of mineral fertilisers as an NPK reference to the soil before sowing winter rape (Brassica napus) in the field experiment.
Laccase production and metabolic diversity among Flammulina velutipes strains (2015)
Twelve Flammulina velutipes strains originating from Poland were identified using internal transcribed spacer (ITS) region sequencing. Based on the sequences obtained, the genomic relationship of the analyzed strains was determined.
Microbiological quality of milk sold directly from producers to consumers (2015) Info PL
The aim of this study was to determine the microbiological quality of raw cow milk from direct sale points. Raw cow milk samples were collected from 5 randomly selected direct sale points for microbiological evaluation.
The Influence of Ecological and Conventional Plant Production Systems on Soil Microbial Quality under Hops (Humulus lupulus) (2014)
The knowledge about microorganisms—activity and diversity under hop production is still limited. We assumed that, different systems of hop production (within the same soil and climatic conditions) significantly influence on the composition of soil microbial populations and its functional activity (metabolic potential).
The Application of the Biolog EcoPlate Approach in Ecotoxicological Evaluation of Dairy Sewage Sludge (2014)
An increasing amount of sewage sludge requires reasonable management, whereas its storage might be environmentally hazardous. Due to the organic matter and nutrient presence in sediments, it may be used as organic fertilizer. However, beyond the valuable contests, sewage sludge can also contain toxic or dangerous ingredients like heavy metals.
Soil Microbial Functional and Fungal Diversity as Influenced by Municipal Sewage Sludge Accumulation (2014)
Safe disposal of municipal sewage sludge is a challenging global environmental concern. The aim of this study was to assess the response of soil microbial functional diversity to the accumulation of municipal sewage sludge during landfill storage.
Community Level Physiological Profiles (CLPP), Characterization and Microbial Activity of Soil Amended with Dairy Sewage Sludge (2012)
The aim of the present work was to assess the influence of organic amendment applications compared to mineral fertilization on soil microbial activity and functional diversity. The field experiment was set up on a soil classified as an Eutric Cambisol developed from loess (South-East Poland).