Antimicrobial Susceptibility Testing

Antibiotic discs, prepared culture media for susceptibility testing (AST) and chromogenic media for detection of resistances mechanisms: KPC, ESBL, VRE, MRSA, produced by BioMaxima, received the expert report of the National Referential Center for Antibiotic Sensitivity of Microorganisms (KORLD)

Antibiotic discs acc. EUCAST v. 10.0 from 01.01.2020

The Antibiotic Paper Discs are manufactured in a modern facility, equipped with automated production lines based on the latest technologies. Each delivery is accompanied by a certificate of quality control. The discs are packed in airtight packaging, type of tube or blister with a desiccant. Production is carried out in accordance with Quality Management System ISO 9001 and ISO 13485. The products are registered and CE marked.

Products
Amikacin 30 Chloramphenicol 30 Norfloxacin 10
Amoxicillin 10 Ciprofloxacin 5 Ofloxacin 5
Amoxicillin-clavulanic acid 2/1 Clindamycin 2 Oxacillin 1
Amoxicillin-clavulanic acid 20/10 Colistin 10 Pefloxacin 5
Ampicillin 2 Daptomycin 30 Phenoxymethylpenicillin 10
Ampicillin 10 Ertapenem 10 Piperacillin 30
Ampicillin-sulbactam 10/10 Erythromycin 15 Piperacillin-tazobactam 30/6
Azithromycin 15 Fosfomycin 200 Quinupristin-dalfopristin 15
Aztreonam 30 Fusidic Acid 10 Rifampicin 5
Benzylpenicillin 1 Gentamicin 10 Streptomycin 300
Cefaclor 30 Gentamicin 30 Teicoplanin 30
Cefadroxil 30 Imipenem 10 Telithromycin 15
Cefalexin 30 Levofloxacin 5 Tetracycline 30
Cefepime 30 Linezolid 10 Ticarcillin 75
Cefixime 5 Mecillinam 10 Ticarcillin-clavulanic acid 75/10
Cefotaxime 5 Meropenem 10 Tigecycline 15
Cefoxitin 30 Minocycline 30 Tobramycin 10
Cefpodoxime 10 Moxifloxacin 5 Trimethoprim 5
Ceftazidime 10 Mupirocin 200 Trimethoprim-sulfamethoxazole 25
Ceftibuten 30 Nalidixic acid 30 Vancomycin 5
Ceftriaxone 30 Netilmicin 10  
Cefuroxime 30 Nitrofurantoin 100

Discs are packed into tight, humidity proof packaging as:

  • blister 1 x 50 discs
  • carton box 5 x 50 discs – 5 blisters per carton box
  • Tube 5 x 50 discs – 5 vials per tube

Blisters and tubes have the humidity absorbers inside, which provide correct stability of antibiotic discs.

 

 

For our antibiotic discs, there is a table prepared, including target values and permissible values range of inhibition zones for reference strains, based on EUCAST v. 10.0, dated 01.01.2020. Part of the table is shown below:

 

Antimicrobial agent

Disc symbol Disc content

Escherichia coli

ATCC 25922

Pseudomonas aeruginosa

ATCC 27853

Staphylococcus aureus

ATCC 29213

      Inhibition zone Inhibition zone Inhibition zone
      Target1 Range2 Target1 Range2 Target1 Range2
Amikacin AK 30 30 22-23 19-26 22 18-26 21 18-24

DISCS DISPENSER

6-position discs dispenser for placing 6 antibiotic discs on 90 mm Petri dishes,

providing appropriate distance between the discs

 

 

 

 


Prepared culture media for susceptibility testing:

Prepared culture media for susceptibility testing recommended by EUCAST :

Mueller Hinton 2 LAB-AGAR™ (MHA), cat. No PP 1170 -  for: Enterobacterales, Pseudomonas spp. Acinetobacter spp., Stenotrophomonas maltophilia, Acinetobacter spp., Staphylococcus spp., Enterococcus spp.

Mueller Hinton 2 LAB-AGAR™ + NAD + 5% HB (MH-F), cat. No PP 0083 - for Streptococcus groups A, B, C i G, Viridans group Streptococci, Streptococcus pneumonia, Haemophilus spp., Moraxella catarrhalis, Listeria monocytogenes, Pasteurella multocida, Campylobacter jejuni and coli, Corynebacterium spp.

Brucella LAB-AGAR™ + 5% SB + wit. K + Hemine, cat. No PP 0082

Mueller Hinton LAB-AGAR™ + Glucose + Methylene Blue, cat. No PP 0105

Mueller Hinton 2 LAB-AGAR™ + Cloxacillin, cat. No PP 0073

Mueller Hinton LAB-AGAR™ + 4% NaCl + Oxacillin, cat. No PP 0006

Mueller Hinton 2 LAB-AGAR™ + 5% SB, cat. No PP 1172

RPMI MOPS LAB-AGAR™, cat. No PP 0095

Schaedler Anaerobe LAB-AGAR™ + 5% SB + wit. K3, cat. No PP 0003


Reference strains for Routine Internal Quality Control as recommended by EUCAST:

  • MM02-10 Escherichia coli NCTC 12241 / ATCC ® 25922
  • MM24-10 Escherichia coli NCTC 11954 / ATCC ® 35218
  • MM10-10 Pseudomonas aeruginosa NCTC 12903 / ATCC ® 2 7853
  • MM14-10 Staphylococcus aureus NCTC 12973 / ATCC ® 29213
  • MM18-10 Enterococcus faecalis NCTC 12697 / ATCC ® 29212
  • MM95-10 Streptococcus pneumoniae NCTC 12977 /ATCC ® 49619
  • MM37-10 Haemophilus influenzae NCTC 12975 / ATCC ® 49766
  • MM36-10 Campylobacter jejuni NCTC 11351 / ATCC ® 33560
 

Reference strains for Extended Internal Quality Control as recommended by EUCAST:

  • MM83-10 Klebsiella pneumoniae NCTC 13368 / ATCC® 700603 – ESBL producer
  • MM64-10 Staphylococcus aureus (MRSA) NCTC 12493 - Methicillin resistant (MRSA), mecA positive
  • MM52-10 Enterococcus faecalis NCTC 13379 / ATCC® 51299 - vanB positive strain, High-level gentamicin and streptomycin resistant
  • MM81-10 Haemophilus influenzae NCTC 12699 / ATCC® 49247 - Reduced susceptibility to β-lactam agents due to PBP mutations in Haemophilus influenzae 

Chromogenic media for mechanisms of bacterial resistance:

  • Chromogenic Acinetobacter MDR LAB-AGAR™
  • Chromogenic COL-APSE LAB-AGAR™
  • Chromogenic ESBL Mod. LAB-AGAR™
  • Chromogenic KPC LAB-AGAR™
  • Chromogenic MRSA LAB-AGAR™
  • Chromogenic Super Carba LAB-AGAR™
  • Chromogenic VRE LAB-AGAR™